Although this normalization procedure has been used in several studies, the relationship between normalized MFE and length has not yet been investigated in detail. Simple indices, obtained by dividing the MFE by the number of nucleotides, have been used for a direct comparison of the folding stability of RNAs of various sizes. The minimum free energy (MFE) of ribonucleic acids (RNAs) increases at an apparent linear rate with sequence length.
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